Fig. 5

Measurement of reactive oxygen species (ROS). a, b Representative images of flow cytometry (a) and quantitative analysis (b) with respect to ROS release in hypoxic cardiomyocytes by transfection with Ad_TRAP1, COXII_shRNA, or both. n = 3/group. *p < 0.05 vs normoxia group; **p < 0.05 vs hypoxia group and hypoxia+Ad_GFP group, respectively; #p < 0.05 vs hypoxia+Ad_TRAP1 group and hypoxia+Ad_TRAP1+Cont_shRNA group. c, d Representative images of flow cytometry (c) and quantitative analysis (d) of ROS release in hypoxic cardiomyocytes by transfection with TRAP1_shRNA, Ad_COXII, or both. n = 3/group. *p < 0.05 vs normoxia group; **p < 0.05 vs hypoxia group and hypoxia+Cont_shRNA group, respectively; #p < 0.05 vs hypoxia+TRAP1_shRNA group and hypoxia+TRAP1_shRNA+Ad_GFP group, respectively. Data are presented as mean ± SEM. Error bars indicate SEM, p value was analyzed using post hoc Tukey’s tests. The experiment was repeated three times. Ad_TRAP1 adenoviral vector encoding TRAP1, Ad_COXII adenoviral vector encoding COXII, Ad_GFP adenoviral vector encoding green fluorescent protein, TRAP1_shRNA adenoviral shRNA specifically targeting TRAP1, COXII_shRNA adenoviral shRNA specifically targeting COXII, Cont_shRNA adenoviral-mediated expression of scrambled shRNA