Fig. 3

Interleukin (IL)-17A neutralization attenuated intestinal pathological changes and zonula occludens-1 (ZO-1) alteration, suppressed intestinal permeability elevation, and inhibited increases of cytokines. Ileal sections were stained with hematoxylin and eosin (H&E) (a, b, c 200x), and mucosa lensions were scored in each group(d). Representative immunofluorescent images depicting membrane localization of ZO-1 in sham group (e-g), burn group (h-j), and burn+anti-IL-17A antibody group (l-m), red staining indicates ZO-1, and blue staining indicates nuclei (400x). Intestinal permeability was measured by fluorescein isothiocyanate (FITC)-dextran levels and is expressed as optical density (OD) value (mean ± standard error of the mean (SEM)) in per group (n). Cytokine protein levels in ileum of each group were determined by Western blotting (o), and summary of cytokines blots is presented as the ratio of cytokines: β-actin densities (p). Cytokine protein levels in serum of each group were determined by enzyme-linked immunosorbent assay (ELISA), and normalized to pg/ml of total serum volume (q). n = 5 per group. * p< 0.05, **p < 0.01, *** p< 0.001. DAPI 4′,6-diamidino-2-phenylindole