Scratch wound healing of fibroblast confluent monolayers. Cells cultured in 12-well plates were mechanically scratched with a sterile 0.1–10 μL pipette tip, and then allowed to reepithelialize for 24 h at 37°C in the presence of different honeys at 0.1% v/v. One sample was exposed to 20% platelet lysate (PL) as positive control (see text). Upper panel: measurements of wound closure rates expressed as the difference between wound width at 0 and 24 h. Bars represent mean±SD of two independent experiments, each with n = 20. The mean of control has been set to 100. Letters on bars indicate clustering on the base of statistical differences determined by pairwise comparisons with the Tukey’s test. Values labeled with same letters are not statistically different from each other, whereas different letters indicate statistical differences (P < 0.01). Lower panel: micrographs of scratch wounded fibroblasts incubated under control conditions, or in the presence of various honeys at 0.1% (v/v), or of 20% PL, and then stained with blue toluidine and observed at 24 h. Scale bar, 200 μm.