Fig. 3

bFGF increased expression of myogenic markers myogenin and MyHC. a Western blot analysis of myogenin and MyHC protein expression in skeletal muscle tissues of saline group and bFGF group at indicated time. Actin was used as the loading control and for band density normalization. All of the experiments were repeated three times. b, c The optical density analysis of myogenin and MyHC protein. Data are expressed as Mean ± SEM (n = 3). d Photographs showing myogenin immunofluorescence (green, red arrow in merge pictures) on the 7th and 14th days in two groups (×200). e Graph of myogenin immunofluorescence in two groups. Data are expressed as mean ± SEM (n = 6). *P < 0.05 compared with saline group