Skip to main content

Mesenchymal stromal cells-exosomes: a promising cell-free therapeutic tool for wound healing and cutaneous regeneration


Cutaneous regeneration at the wound site involves several intricate and dynamic processes which require a series of coordinated interactions implicating various cell types, growth factors, extracellular matrix (ECM), nerves, and blood vessels. Mesenchymal stromal cells (MSCs) take part in all the skin wound healing stages playing active and beneficial roles in animal models and humans. Exosomes, which are among the key products MSCs release, mimic the effects of parental MSCs. They can shuttle various effector proteins, messenger RNA (mRNA) and microRNAs (miRNAs) to modulate the activity of recipient cells, playing important roles in wound healing. Moreover, using exosomes avoids many risks associated with cell transplantation. Therefore, as a novel type of cell-free therapy, MSC-exosome -mediated administration may be safer and more efficient than whole cell. In this review, we provide a comprehensive understanding of the latest studies and observations on the role of MSC-exosome therapy in wound healing and cutaneous regeneration. In addition, we address the hypothesis of MSCs microenvironment extracellular vesicles (MSCs-MEVs) or MSCs microenvironment exosomes (MSCs-MExos) that need to take stock of and solved urgently in the related research about MSC-exosomes therapeutic applications. This review can inspire investigators to explore new research directions of MSC-exosome therapy in cutaneous repair and regeneration.


The skin is frequently damaged as a result of acute or chronic wounds such as extensive burns, trauma or ulcers of various aetiology. These injuries not only destroy the barrier function of the skin but also modify the sensory perceptions of temperature, pain and touch [1]. Besides, they constitute a painful experience for patients both physically and mentally, and also cause a huge socioeconomic burden [2]. Therefore, identifying an effective approach to accelerate cutaneous regeneration and restore the functions of the injured skin is an urgent requirment. Besides, this topic has become a significant challenge in plastic and reconstructive surgery. A novel therapy for wound healing and regeneration gaining momentum in the past few years has been the use of mesenchymal stromal cells (MSCs). MSCs reside in normal skin and play a critical role in wound healing. Therefore, the application of exogenous MSCs was proposed to promote regenerative healing of wounded skin [3]. Although great progress has been made in the application of MSCs in wound repair and cutaneous regeneration, the limitations inherent to MSCs cell therapy cannot be ignored. Therefore, the exosomes derived from MSCs (MSC-exosomes) have gained much attention as a new potential “cell-free” approach in the field of wound healing and cutaneous regeneration.

In this review, we discuss the confusing issues related to MSCs, i.e. the lack of universally accepted criteria for defining the MSCs phenotype and/or their functional properties, and discuss the current clinical trials using MSCs in wound healing therapy and their current limitations. Then we expound the roles of MSC-exosomes in cutaneous regeneration and summarize their underlying molecular mechanisms. We also clarify the current scientific problems of MSC-exosomes as the research trend of “cell-free” therapy. Finally, we bring forward the key problems which at present need to be solved and express the hope that more researchers will study this issue in depth.


The defining and naming of MSCs

Although the biological characteristics and therapeutic potential of MSCs have been deeply studied, the behavior in vivo and developmental origin of MSCs have not been clarified yet. With regard to the source of MSCs, since mesenchymal stem cells exist in almost all tissues and organs, there is also a popular hypothesis that MSCs come from perivascular cells [4]. But some researchers have found that MSCs can also come from tissues without blood vessels and nerves, such as intervertebral disc [5]. Precisely defining MSCs has been a challenge, as the field is complicated by inconsistencies related to MSCs nomenclature and identification criteria. For example, bone marrow stromal cells, bone marrow stromal stem cells, MSCs, mesenchymal stem cells, and medical signal cells, all of which describe the same group of cells, the widely recognized the acronym, MSC, may be used for all of them.

Bone marrow stromal cells were first discovered and reported by Friedenstein AJ in the late 1960s. In 1988, Owen et al. defined it as bone marrow stromal stem cells on the basis of previous researchers’ work. In 1991, Caplan et al. found that there was a group of mesoderm-derived stem cells with different tissue lines in bone marrow during embryonic development, and named these cells as mesenchymal stem cells. The name has been widely recognized in the world [6]. However, some scholars believe that there are no sufficient evidences of self-renewal and multidirectional differentiation potential ability of mesenchymal stem cells in vivo, and their biological characteristics do not meet the standards of stem cells. It is not scientific enough to call stem cells, which may mislead patients with excessive and unrealistic expectations. Therefore, Horwitz called for the naming of these cells as “MSCs” at the International Conference on Cell Therapy [7]. The International Society for Cellular Therapy also tried to resolve challenges in confirming MSC identity by proposing three minimal criteria for defining human MSCs: (1) the cells must be plastic-adherent when maintained in standard culture conditions using tissue culture flasks; (2) ≥95% of the population must express CD105, CD73, and CD90 and ≤ 2% must not express CD45, CD34, CD14, CD11b, CD79α, or CD19, and HLA class II surface molecules; and (3) the cells must be able to differentiate into osteoblasts, adipocytes, and chondroblasts under standard in vitro differentiating conditions [8]. Furthermore, the researchers found that the conditioned medium of MSCs had biological effects equivalent to MSCs in promoting wound healing and preventing and treating organ fibrosis. It was suggested that the biological effect of MSCs mainly came from its paracrine mechanism. It was found that MSCs extracellular vesicles or exocrine bodies also had almost equivalent biological effects to MSCs. In 2010, Caplan proposed the new name of “medicinal signal cell, MSC” , because of these cells like “medicine” homing to the site of injury or disease, secreting bioactive factors, playing a treatment in situ [9]. In fact, no matter how precisely MSCs are defined, this does not affect the applicative prospects of these cells in treatment.

MSCs in wound healing and cutaneous regeneration

Cutaneous wound healing is a highly organized physiological process that restores the integrity of the skin following injury. It involves the interplay between various populations of cells and is typically categorized into three reciprocally overlapping phases: inflammation, proliferation, and maturation [10, 11]. Various MSCs are also deemed to partake in this process, like the endogenous cutaneous MSCs which include dermal papilla cells (DPCs) and the dermal sheath cells (DSCs) [12,13,14]. And the perivascular pericytes and MSCs residing in the adipose tissue may act as MSCs in vivo [15,16,17]. However, in most trauma cases, such as deep burns and chronic ulcers, there are not enough endogenous cutaneous MSCs to partake in wound self-repair because of the missing dermal tissue. Therefore, exogenous MSCs have been applied to wounds to exploit their physiological therapeutic actions in wound healing. Regardless of the caveats in their identity or source, MSCs were reported to exert beneficial effects on both wound healing and scarring. Since MSCs are endowed with a wide differentiation potential, they are attractive treatment options in regenerative medicine and, over the past decade, have rapidly emerged as a cell therapeutic tool for advancing wound healing.

Most of the evidence related to MSCs activity in wound healing comes from bone marrow-derived MSCs (BMSCs) and other tissue of origin MSCs used in animal models, with only a small number of published clinical studies. In addition, these clinical studies are based on the transplantation of autologous BMSCs [18,19,20,21]. BMSCs are collected via bone marrow aspiration, which is a safe but painful and invasive procedure, sometimes associated with complications such as infection and hemorrhage [22]. Additionally, bone marrow is a limited resource; there occurs an age-dependent reduction in bone marrow cells numbers [23]. Therefore, MSCs of several different sources have been used as alternative choices, including adipose-derived stromal cells (ADSCs), dermal MSCs, and MSCs from amniotic fluid and umbilical cord. ADSCs and dermal MSCs are abundantly available in fat and skin tissues, can be harvested with minimally invasive procedures, and their use is devoid of ethical controversies making them good alternatives to BMSCs. ADSCs and dermal MSCs have similar biological characteristics, immunogenicity, and potential to differentiate to BMSCs [24,25,26,27,28,29] . In clinical trials currently under evaluation [30], ADSCs have demonstrated a therapeutic potential in burn wounds and ulcers. Likewise, dermal MSCs have shown beneficial effects on wound healing in clinical trials [31, 32]. Although, there are no clinical trials related to human amniotic membrane-derived MSCs (hAM-dMSCs) and human umbilical cord-derived MSCs (hUC-dMSCs) in wound healing have appeared currently. These promising clinical studies indicate that MSC-based therapies are safe and potentially efficacious, with no indication that any particular MSC tissue origin has an advantage for wound healing over the others [12].

Current limitations of MSCs application in wound healing

Despite progression in MSC-based therapies, many challenges to overcome before MSCs can be used for effectively for wound healing treatment. The drawbacks in the clinical application of MSCs have emerged gradually: Firstly, there is considerable heterogeneity in the delivery protocols, wound models, and MSCs populations among published studies which makes it hard to determine the impact of timing of delivery, number of cells delivered, and site of delivery on MSCs engraftment upshots [33]. Secondly, there is no evidence that MSCs differentiate into phenotypes typical of resident cutaneous cells during skin wound healing [34]. Increasing evidences suggest that MSCs secrete bioactive factors through endocrine and paracrine pathways to target tissues and cells, thereby reducing wound inflammation and promoting tissue repair [33]. So the criteria to assess the need for direct MSCs transplantation onto wounds are to be determined. Finally and importantly, current challenges with the use of MSCs concern the lack of universally accepted criteria for defining the MSCs phenotype or their functional properties, and further clinical trials are needed to demonstrate the potential therapeutic benefits of MSCs in vast cohorts of patients [33].

Exosomes derived from MSCs (MSC-exosomes)

Exosomes are cell-derived vesicles that are present in many and perhaps all eukaryotic fluids, including blood, urine, and growth media of cell cultures [35]. The reported diameter of exosomes is between 30nm and 100 nm, which is larger than low-density lipoproteins (LDL) but much smaller than, for example, other vesicles or red blood cells. Exosomes are either released from the cell when multivesicular bodies fuse with the plasma membrane or released directly from the plasma membrane [36]. Reportedly, the contents within exosomes are manifold including cytokines, proteins, lipids, mRNAs, miRNAs, noncoding RNAs (ncRNAs), ribosomal RNAs (rRNAs), cytokines and chemokines [37]. Accumulating lines of evidence shown that exosomes have specialized functions and play keys role in processes such as coagulation, intercellular signaling, and waste products management [38]. Consequently, the interest in the clinical applications of exosomes is growing. Their functions include immune regulation, vascular regeneration promoting, mediation of cell proliferation, differentiation, migration and apoptosis, preserving the body physiological condition, and partaking in disease processes [39].

MSC-derived exosomes (MSC-exosomes) exhibit the characteristics of resource cells, which can promote cell self-repair and tissue regeneration, restore tissue homeostasis and accelerate wound repair in injury areas [40]. In recent years, some studies have suggested that MSCs have a strong ability to produce exosomes [41]. Most researchers believe that MSC-exosomes are the main effective paracrine component of MSCs and play biological effect almost equivalent to those of whole MSCs. In comparison with MSCs, MSC-exosomes have the following advantages: First, MSC-exosomes exert intense biological effects because they directly fuse with target cells. Second, MSC-exosomes can be stored and transported at − 70 °C for a long time since their effective components are protected by the exosome’s plasma membrane, which is not easy to be destroyed. Third, the concentration, dose, route and time of use are easy to control. Last but not least, there is no risk of immune rejection and tumorigenesis caused by cell transplantation therapy [42].

MSC-exosomes in wound healing and cutaneous regeneration

The typical skin regeneration process can be summarized as three overlapping stages: inflammation phase, proliferation phase (cell proliferation and reepithelization) and remodeling phase [10,11,12]. Currently, all the researches on MSC-exosomes in wound healing and cutaneous regeneration are also focussed on the role of MSC-exosomes in the above three stages.

Mechanism of MSC-exosomes behavior on inflammation phase

Inflammation is a body self-defense mechanism in response to harmful stimuli, and an acute and well-regulated inflammatory response is beneficial for normal wound healing [43]. By contrast, a chronic and dysregulated inflammatory response may delay wound healing and promote fibrosis, excessive scar formation or inhibition of re-epithelialization [44]. Macrophages are prominent inflammatory cells which play an important role in the cutaneous regeneration process. Recent evidence has suggested that macrophages influence each stage of cutaneous regeneration and present a proinflammatory M1 phenotype and an anti-inflammatory M2 phenotype. Macrophage dysfunction may promote excessive inflammation or fibrosis [45]. MSC-derived extracellular vesicles, including MSC-exosomes, promote the significant switching of recipient’s macrophages toward the anti-inflammatory M2 phenotype [46]. In addition, MSC-exosomes can regulate the activation, differentiation, and proliferation of B lymphocytes and can also suppress T-lymphocyte proliferation. MSC-exosomes can convert activated T lymphocytes into the T-regulatory phenotype, thereby exerting immunosuppressive effects [47, 48]. Furthermore, the regulation of inflammatory factors plays an important role in skin tissue regeneration, and excessive production of cytokines may lead to tissue injury [49]. Exosomes derived from different kinds of MSCs can mitigate the inflammatory response caused by multiple stimuli through the down-regulation of proinflammatory enzymes, like inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and of cytokines and chemokines, like tumor necrosis factor (TNF)-α, interleukin (IL)-1β and monocyte chemoattractant protein (MCP)-1. Moreover, in many disease models, MSCs-exosomes can drive the up-regulation of an anti-inflammatory cytokine, i.e. IL-10, which reportedly plays a critical role in the control of cutaneous wound inflammation and scar formation [50,51,52]. Various studies have revealed that MSC-exosomes exert immunomodulatory effects through specific miRNAs. By analyzing miRNA expression profiles, they showed that hUC-dMSCs exhibit the highest content of three miRNAs (miRNA- 21, miRNA-146a and miRNA-181c) specifically related to the regulation of immune response and inflammation [53, 54]. Furthermore, other studies showed that hUC-dMSCs-exosomes carrying miRNA-181c attenuated the burn-induced very intense inflammation by down-regulating the Toll-like receptor 4 (TLR4) signaling pathway [51]. Anti-inflammatory miRNAs (miRNA-124a, miRNA-125b) detected within exosomes have been implicated in the post-transcriptional silencing of chemokines and cytokines (e.g., TNF-α and MCP-1) which instead contribute to the persistence of inflammatory cellular infiltrations in wound healing [55, 56]. Overall, the specific molecular mechanisms through which MSC-exosomes inhibit inflammation in the setting of wound healing and cutaneous regeneration also need to be clarified by further studies.

Mechanism of MSC-exosomes behavior on proliferation phase

In the proliferation phase, neoangiogenesis, collagen deposition, granulation tissue formation, re-epithelialization, and wound contraction concur [57]. The formation of neoangiogenesis is a crucial step in various physiological and pathological processes, including wound healing and tissue repair [10, 58, 59]. MSC-exosomes are enriched in various angiogenesis-related proteins and RNAs, including miRNAs that could activate multiple signaling pathways in endothelial cells. The same exosomes can induce the expression of numerous trophic factors [60]. In addition, Li et al.revealed that transplanted human umbilical cord blood endothelial progenitor cells-derived exosomes (EPC-exosomes) could upregulate the expression of angiogenesis-related molecules, including vascular endothelial growth factor (VEGF) - A, vascular endothelial growth factor receptor (VEGFR)-2, fibroblast growth factor (FGF)- 1, E-selectin, angiopoietin-1, Chemokine (C-X-C motif) ligand 16, IL- 8, and endothelial nitric oxide synthase (eNOS), in vascular endothelial cells [61]. Furthermore, the mRNA levels of matrix metalloproteinase (MMP)-9 were remarkably decreased in endothelial cells stimulated with EPC-exosomes [61]. The study has found that significantly higher expression of MMP-9 was associated with poor wound healing [62]. Thus, the proangiogenesis effects of EPC-exosomes may be partially attributed to their inhibition of MMP-9. However, it is still necessary to reveal their further mechanisms about angiogenesis in wound repair.

Cell proliferation and skin re-epithelization are crucial for cutaneous regeneration. Skin fibroblasts play a relevant role in skin tissue repair and regeneration: they participate in wound contraction, extracellular matrix deposition, tissue remodeling, and so on [63]. MSC-exosomes can be internalized and hence transport their contents, such as proteins and RNAs, into the receiving cells to regulate their proliferation and migration. It has been proven that MSC-exosomes regulate the proliferation and migration of fibroblasts by modulating the expression of growth factors and their related genes [64,65,66] thus taking part to the formation of granulation tissue and to the synthesis of collagen which provides structural support for wound repair [67, 68]. Some scholars treated fibroblasts extracted from chronic diabetic ulcer wounds with exosomes derived from BMSCs. The results showed that exosomes could promote the proliferation and migration of fibroblasts in a dose-dependent manner. Human fibrocyte-derived exosomes contain proteins and miRNAs with diverse biological activities, and these exosomes accelerated wound healing by inducing the migration and proliferation of skin cells in the diabetic rat model [55].

In addition, the study found that exosomes derived from hUC-dMSCs promoted the proliferation of skin cells (dermal fibroblasts, epidermal keratinocytes) in a dose-dependent manner. In in vivo experiments, the researchers used local multi-point injection to evaluate the effect of exosomes by injecting exosomes around deep II degree burn wounds models. The results showed that exosomes derived from hUC-dMSCs accelerated wound healing, promoted re-epithelialization, and increased the expression of cytokeratin-19 (CK19), proliferating cell nuclear antigen (PCNA) and collagen I [69, 70]. Moreover, transplanting human amniotic epithelial cell-derived exosomes (hAEC-exosomes) to wound sites accelerated wound closure and re-epithelization [71].

Mechanism of MSC-exosomes behavior on remodeling phase

Extracellular matrix (ECM) mainly constituents of four kinds of substances, namely collagen, non-collagen (fibronectin, laminin), elastin, proteoglycans, and aminoglycans. The key to ECM reconstruction is the synthesis and degradation of collagen. Insufficient or excessive ECM formation can cause the wound surface not to heal or scar formation. MSC-exosomes have been shown to regulate ECM re-synthesis in addition to participating in the above cellular effects. Reportedly, exosomes derived from hUC-dMSCs can promote the de novo synthesis of type I collagen and elastin [65], and exosomes from the inducing process which human induced pluripotent stem cells (hiPSCs) had differentiated to dermal MSCs (dMSCs) can promote the synthesis of type I collagen, type III collagen, and elastin proteins, and enhance the expression levels of type I collagen, type III collagen, and elastin mRNAs [72]. These findings prove that MSC-exosomes can promote the regeneration of ECM and thus advance wound healing. The exosomes derived from ADSCs (ADSCs-exosomes) can also regulate collagen synthesis at different stages of wound healing, accelerate wound healing through an early stage increase in the production of type I and type III collagen, and inhibit collagen synthesis in a late stage, thereby reducing scar formation [73]. In a skin-defect mouse model, exosomes derived from hUC-dMSCs did prevent scar formation by inhibiting the differentiation of fibroblasts into myofibroblasts [74]. Moreover, the research reported that hAEC-exosomes exerted beneficial effects in a rat model of scarless wound healing. High concentration of hAEC-exosomes partly reduced ECM deposition by stimulating the expression of MMP-1 [71]. In addition, Wang et al. posited that ADSCs-exosomes can inhibit the formation of type III/type I collagen by directly acting on fibroblasts [75]. In a study applying traceable ADSCs-exosomes to repair skin defects in mice, the exosomes were recruited around the skin wounds to perform their functions and to increase the rate of wound healing. Histological analysis showed that exosomes could promote collagen synthesis in the early stage of wound healing, and inhibit collagen synthesis in the late stage to inhibit scar tissue formation [64] .All these studies indicate that MSC-exosomes play a key role in ECM remodeling, which is also the possible mechanism of reducing scar formation.

Hypothesis of MSCs-MEV or MSCs-MExos and future perspectives

Over the past decades, a deep-going research has taken place concerning MSCs and MSC-exosomes roles in the field of wound repair and cutaneous regeneration. MSC-exosomes based therapy is emerging as a promising technique able to promote wound healing and minimize scarring. Accordingly, being a cell-free alternative therapy, MSC-exosomes enjoy many advantages, being easy to be prepared, stored and transported, easy to be dosed, and easy to be administered at the time of choice. They also appear to have a high therapeutic efficiency, and to carry no risk of immune rejection and tumorigenesis. Thus, MSC-exosomes are endowed with a remarkable potential for cutaneous regeneration and could effectively replace whole MSCs-based therapy. The molecular mechanisms through which the MSC-exosomes bring into effect cutaneous regeneration will be fully brought to light by further studies looking into their specific contents and functions. At present, most of the mechanisms discussed were studied in rodents, but animal physiology cannot always be extrapolated to humans. So further clinical trials using exosomes of human origin are needed to definitively demonstrate the skin regenerative therapeutic potential of MSCs in a large number of patients. However, there are still key scientific issues that need to be resolved before.

Although MSCs derived from different tissues do not have the same differentiation potency, their physical and biological functions show a high level of consistency [76, 77]. So it might not be considered as an obstacle that which MSCs should be chosen to produce exosomes. However, MSCs transplantation has different biological effects in different stages of wound healing or in different diseases. In fact, the fate and paracrine effects of MSCs are closely related to the microenvironment of their transplantation site [78, 79]. For instance, studies have shown that transplantation of MSCs into organs and tissues can up-regulate the expression of VEGF and accelerate the regeneration and healing of injured tissues [80]; But in some studies concerning tumors, researchers have found that MSCs can down-regulate the expression of VEGF and inhibit the angiogenesis supporting the growth of tumors [81, 82]. Our studies also corroborate that MSCs derived from different sources can promote fibroblast proliferation and migration through the paracrine effect, and up-regulate the expression of transforming growth factor (TGF) -β1, collagen I and angiogenesis factors in fibroblasts and wound tissues to advance the formation of granulation tissue and accelerate wound re-epithelialization. But when MSCs were transplanted after wound re-epithelialization, the biological effects were changed by the paracrine effect in in vivo and in vitro studies (e.g., the expression of fibrosis-related factors such as TGF-β1, α-smooth muscle actin (α-SMA) and collagen I was down-regulated, and the expression of anti-fibrosis factors such as TGF-β3 and Decorin was up-regulated to hinder the formation of the scar.

Recent research also has shown that stimulating hUC-dMSCs with interferon (IFN) -γ if there were in a simulated inflammatory microenvironment can significantly increase the release of MSCs-exosomes. IFN-γ can enhance the immune regulatory activity of hUC-dMSCs acting like a “license” factor and increase the proportion of CD4+, CD25+ and Foxp3+ T cells among the whole Regulatory T cells (Tregs) [83]. Lipopolysaccharide (LPS) pretreated hUC-dMSCs secrete more proteins than untreated ones, and the exosome derived from LPS-pretreated hUC-dMSCs are rich in miRNA let-7b, which can induce macrophages to take on the anti-inflammatory M2 phenotype [53]. (Fig. 1). In addition, the research showed that 1–2% hypoxia environment, media with TNF-α, and three-dimensional culture system all had been found significantly change the components of MSCs paracrine factors in vitro culture [84]. (Fig. 2) The release and components of MSC-exosomes can be modulated by changing the cell culture conditions, that is the microenvironment in which the cells live.

Fig. 1
figure 1

Under the stimulating human umbilical cord-derived mesenchymal stromal cells (hUC-dMSCs) with different factors, the quantities and components of exosomes had changed. Then, the biological characteristics of target cells also have changed after received the exosomes. (a) In a normal situation, target cells receive microvesicles and exosomes released from MSCs. (b) Stimulating hUC-dMSCs with interferon (IFN)- g, there were in a simulated inflammatory microenvironment that can significantly increase the release of MSCs-exosomes. Then, it can enhance the immunomodulatory activity of hUC-discs and increase the proportion of CD4+, CD25+ and Foxp3+T cells in the entire regulatory T cells. (c) Stimulating hUC-dMSCs with lipopolysaccharide (LPS) can secrete more exosomes than untreated ones, and the exosome derived from them are rich in miRNA let-7b, which can induce macrophages to exhibit the anti-inflammatory M2 phenotype by the specific signal pathway

Fig. 2
figure 2

With the changing of culture environment, the components of mesenchymal stromal cell - extracellular vesicles (MSCs-EVs) or MSCs-exosomes also have changed. IFN interferon, IL interleukin, TGF transforming growth factor, TNF , tumor necrosis factor, VEGF vascular endothelial growth factor, α-SMA α-smooth muscle actin

However, all the current studies on the mechanisms of MSC-exosomes activities are based on the MSC-exosomes isolated from the standard culture medium in vitro. This obviously contradicts the important scientific argument that the paracrine effect of MSCs is closely related to their microenvironment. In other words, MSC-exosomes secreted in different microenvironments have different components which exert dissimilar biological effects. This important scientific issue has been ignored by almost all researchers of studying MSCs-EVs or MSC-exosomes.


Wound healing and cutaneous regeneration is a complicated and dynamic process, and the same is true of other diseases. If we only isolate MSC-exosomes from conditioned medium supernatants under standard laboratory culture conditions, then use them as a cell-free therapy for different diseases or different pathological stages of the same disease, it will be obviously difficult to “accurately” achieve an effective cell therapy based on MSCs.

Even if, we can not fully elucidate its essence and mechanism of inducing MSCs paracrine effect, and it is difficult or even impossible to simulate the microenvironment completely at present. However, we can imitate it in the vitro culture systems which are based on the known environmental factors, such as inflammatory factors in wound inflammation stage, and hypoxia factors in ischemic anoxic wounds. There is no doubt, the complexity of wound microenvironment should not be the excuse of ignoring the microenvironmental factors in studying MSCs-EVs or MSCs-Exos.

Therefore, we propose the hypothesis of MSCs-MEV or MSCs-MExos which takes stock of the conditions in which the exosomes were produced as related to their therapeutic applications. We also hope that more scholars will pay a greater attention to this scientific problem, which is to find more and more suitable methods simulating the damaged site microenvironment of diverse diseases or of different pathological stages of the same illness. If these key problems can be solved, MSCs will have a significant impact on wound healing and cutaneous regeneration, besides other diseases, as a cell-free therapy.

Availability of data and materials

Not applicable.



Adipose-derived stromal cells


Exosomes derived from ADSCs


Bone marrow-derived MSCs






Dermal mesenchymal stromal cells


Dermal papilla cells


Dermal sheath cells


Extracellular matrix


Endothelial nitric oxide synthase


Endothelial progenitor cells-derived exosomes


Fibroblast growth factor


Human amniotic epithelial cell-derived exosomes


Human amniotic membrane-derived mesenchymal stromal cells


Human umbilical cord-derived mesenchymal stromal cells


Human induced pluripotent stem cells






Inducible nitric oxide synthase


Low-density lipoproteins




Monocyte chemoattractant protein




Matrix metalloproteinase


Messenger RNA


Exosomes derived from MSCs


Mesenchymal stromal cells


MSCs microenvironment extracellular vesicles


MSCs microenvironment exosomes


noncoding RNAs


Proliferating cell nuclear antigen


Transforming growth factor


Tumor necrosis factor


T cells


Vascular endothelial growth factor


Vascular endothelial growth factor receptor

X-C motif:



α-smooth muscle actin


  1. Blais M, Parenteau-Bareil R, Cadau S, Berthod F. Concise review: tissue-engineered skin and nerve regeneration in burn treatment. Stem Cells Transl Med. 2013;2:545–51.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Rani S, Ritter T. The exosome-a naturally secreted nanoparticle and its application to wound healing. Adv Mater. 2016;28:5542–52.

    Article  CAS  PubMed  Google Scholar 

  3. Yamaguchi Y, Yoshikawa K. Cutaneous wound healing: an update. J Dermatol. 2001;28:521–34.

    Article  CAS  PubMed  Google Scholar 

  4. Caplall AI. New MSC: MSCs as pericytes are sentinels and gatekeepers. J Orthop Res. 2017;35(6):1151–9.

    Article  Google Scholar 

  5. Blanco JF, Graciani IF, Sanchez-Guijo FM,  Muntión S, Hernandez-Campo P, Santamaria C, et al. Isolation and characterization of mesenchymal stromal cells from human degenerated nucleus pulposus: comparison with bone marrow mesenchymal stromal cells from the same subjects. Spine (Phila Pa 1976). 2010;35(26):2259–65.

    Article  PubMed  Google Scholar 

  6. Caplan AI. Mesenchymal stem cells. J Orthop Res. 1991;9(5):641.

    Article  CAS  PubMed  Google Scholar 

  7. Bianco P, Robey PG, Simmons PJ. Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell. 2008;2(4):313–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Dominici M, le Blanc K, Mueller I,  Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defifining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8(4):315–7.

    Article  CAS  PubMed  Google Scholar 

  9. Al C. Mesenchymal stem cells: time to change the name! Stem Cells Transl Med. 2017;6(6):1445–51.

    Article  Google Scholar 

  10. Martin P. Wound healing–aiming for perfect skin regeneration. Science. 1997;276(5309):75–81.

    Article  CAS  PubMed  Google Scholar 

  11. Singer AJ, Clark RAF. Cutaneous wound healing. N Engl J Med. 1999;341(10):738–46.

    Article  CAS  PubMed  Google Scholar 

  12. Maxson S, Lopez EA, Yoo D, Danilkovitch-Miagkova A, LeRoux MA. Concise review: role of mesenchymal stem cells in wound repair. Stem Cells Transl Med. 2012;1(2):142–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Jahoda CAB, Reynolds AJ. Hair follicle dermal sheath cells: unsung participants in wound healing. Lancet. 2001;358(9291):1445–8.

    Article  CAS  PubMed  Google Scholar 

  14. Toma JG, McKenzie IA, Bagli D, Miller FD. Isolation and characterization of multipotent skin-derived precursors from human skin. Stem Cells. 2005;23(6):727–37.

    Article  CAS  PubMed  Google Scholar 

  15. Bodnar RJ, Satish L, Yates CC, Wells A. Pericytes: a newly recognized player in wound healing. Wound Repair Regen. 2016;24(2):204–14.

    Article  PubMed  PubMed Central  Google Scholar 

  16. Paquet-Fifield S, Schlüter H, Li A,  Aitken T, Gangatirkar P, Blashki D, et al. A role for pericytes as microenvironmental regulators of human skin tissue regeneration. J Clin Invest. 2009;119(9):2795–806.

  17. Schmidt BA, Horsley V. Intradermal adipocytes mediate fibroblast recruitment during skin wound healing. Development. 2013;140(7):1517–27.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Fathke C, Wilson L, Hutter J, Kapoor V, Smith A, Hocking A, et al. Contribution of bone marrow–derived cells to skin: collagen deposition and wound repair. Stem Cells. 2004;22(5):812–22.

    Article  PubMed  PubMed Central  Google Scholar 

  19. Badiavas EV, Falanga V. Treatment of chronic wounds with bone marrow–derived cells. Arch Dermatol. 2003;139(4):510–6.

    Article  PubMed  Google Scholar 

  20. Falanga V, Iwamoto S, Chartier M, Yufit T, Butmarc J, Kouttab N, et al. Autologous bone marrow-derived cultured mesenchymal stem cells delivered in a fibrin spray accelerate healing in murine and human cutaneous wounds. Tissue Eng. 2007;13(6):1299–312.

    Article  CAS  PubMed  Google Scholar 

  21. Yoshikawa T, Mitsuno H, Nonaka I,  Sen Y, Kawanishi K, Inada Y, et al. Wound therapy by marrow mesenchymal cell transplantation. Plast Reconstr Surg. 2008;121(3):860–77.

    Article  CAS  PubMed  Google Scholar 

  22. Kita K, Gauglitz GG, Phan TT, Herndon DN, Jeschke MG. Isolation and characterization of mesenchymal stem cells from the sub-amniotic human umbilical cord lining membrane. Stem Cells Dev. 2010;19(4):491–502.

    Article  CAS  PubMed  Google Scholar 

  23. Rao MS, Mattson MP. Stem cells and aging: expanding the possibilities. Mech Ageing Dev. 2001;122(7):713–34.

    Article  CAS  PubMed  Google Scholar 

  24. Zuk PA, Zhu M, Mizuno H,  Huang J, Futrell JW, Katz AJ, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7(2):211–28.

    Article  CAS  PubMed  Google Scholar 

  25. Richardson GD, Arnott EC, Jenna Whitehouse C, Lawrence CM, Hole N, Jahoda CAB. Cultured cells from the adult human hair follicle dermis can be directed toward adipogenic and osteogenic differentiation. J Invest Dermatol. 2005;124(5):1090–1.

    Article  CAS  PubMed  Google Scholar 

  26. Lu F, Li J, Gao J, Ogawa R, Ou C, Yang B, et al. Improvement of the survival of human autologous fat transplantation by using VEGF-transfected adipose-derived stem cells. Plast Reconstr Surg. 2009;124(5):1437–46.

    Article  CAS  PubMed  Google Scholar 

  27. Puissant B, Barreau C, Bourin P, Clavel C, Corre J, Bousquet C, et al. Immunomodulatory effect of human adipose tissue-derived adult stem cells: comparison with bone marrow mesenchymal stem cells. Br J Haematol. 2005;129(1):118–29.

    Article  PubMed  Google Scholar 

  28. Ma D, Kua JEH, Lim WK, Lee ST, Chua AWC. In vitro characterization of human hair follicle dermal sheath mesenchymal stromal cells and their potential in enhancing diabetic wound healing. Cytotherapy. 2015;17(8):1036–51.

    Article  CAS  PubMed  Google Scholar 

  29. Driskell RR, Clavel C, Rendl M, Watt FM. Hair follicle dermal papilla cells at a glance. J Cell Sci. 2011;124(8):1179–82.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Akita S, Akino K, Hirano A, Ohtsuru A, Yamashita S. Noncultured autologous adipose-derived stem cells therapy for chronic radiation injury. Stem Cells Int. 2010;2010, Article ID 532704:8.

    Article  Google Scholar 

  31. Jiménez F, Garde C, Poblet E,  Jimeno B, Ortiz J, Martínez ML, et al. A pilot clinical study of hair grafting in chronic leg ulcers. Wound Repair Regen. 2012;20(6):806–14.

    Article  PubMed  Google Scholar 

  32. Martínez M-L, Escario E, Poblet E, Sánchez D, Buchón FF,  Izeta A, et al. Hair follicle–containing punch grafts accelerate chronic ulcer healing: a randomized controlled trial. J Am Acad Dermatol. 2016;75(5):1007–14.

    Article  PubMed  Google Scholar 

  33. Hu MS, Borrelli MR, Lorenz HP,  Longaker MT, Wan DC. Mesenchymal stromal cells and cutaneous wound healing: A comprehensive review of the background, role, and therapeutic potential. Stem Cells Int. 2018;2018:6901983.

    Google Scholar 

  34. Javazon EH, Keswani SG, Badillo AT, Crombleholme TM, Zoltick PW, Radu AP, et al. Enhanced epithelial gap closure and increased angiogenesis in wounds of diabetic mice treated with adult murine bone marrow stromal progenitor cells. Wound Repair Regen. 2007;15(3):350–9.

    Article  PubMed  Google Scholar 

  35. Keller S, Sanderson MP, Stoeck A, Altevogt P. Exosomes: from biogenesis and secretion to biological function. Immunol Lett. November 2006;107(2):102–8.

    Article  CAS  PubMed  Google Scholar 

  36. Booth AM, Fang Y, Fallon JK, Yang JM, Hildreth JE, Gould SJ. Exosomes and HIV gag bud from endosome-like domains of the T cell plasma membrane. J Cell Biol. March 2006;172(6):923–35.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Simons M, Raposo G. Exosomes—vesicular carriers for intercellular communication. Curr Opin Cell Biol. 2009;21(4):575–81.

    Article  CAS  PubMed  Google Scholar 

  38. van der Pol E, Böing AN, Harrison P, Sturk A, Nieuwland R. Classification, functions, and clinical relevance of extracellular vesicles. Pharmacol Rev. July 2012;64(3):676–705.

    Article  PubMed  CAS  Google Scholar 

  39. Tkach M, Thery C. Communication by extracellular vesicles:where we are and where we need to go. Cell. 2016;164(6):1226–32.

    Article  CAS  PubMed  Google Scholar 

  40. Lai RC, Yeo RW, Lim SK. Mesenchymal stem cell exosomes. Semin Cell Dev Biol. 2015;40:82–8.

    Article  CAS  PubMed  Google Scholar 

  41. Yeo RW, Lai RC, Zhang B, Tan SS, Yin Y, Teh BJ, et al. Mesenchymal stem cell: an efficient mass producer of exosomes for drug delivery. Adv Drug Deliv Rev. 2013;65(3):336–41.

    Article  CAS  PubMed  Google Scholar 

  42. Lu K, Li HY, Yang K,  Wu JL, Cai XW, Zhou Y, et al. Exosomes as potential alternatives to stem cell therapy for intervertebral disc degeneration: in-vitro study on exosomes in interaction of nucleus pulposus cells and bone marrowmesenchymal stem cells. Stem Cell Res Ther. 2017;8(1):108.

  43. Sugimoto MA, Sousa LP, Pinho V, Perretti M, Teixeira MM. Resolution of inflammation: what controls its onset? FrontImmunol. 2016;7:160.

    Google Scholar 

  44. Landén NX, Li D, Ståhle M. Transition from inflammation to proliferation: a critical step during wound healing. Cell Mol Life Sci. 2016;73:3861–85.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  45. Koh TJ, DiPietro LA. Inflammation and wound healing: the role of the macrophage. Expert Rev Mol Med. 2011;13:e23.

    Article  PubMed  PubMed Central  Google Scholar 

  46. Lo Sicco C, Reverberi D, Balbi C, Ulivi V, Principi E, Pascucci L, et al. Mesenchymal stem cell-derived extracellular vesicles as mediators of anti-inflammatory effects: endorsement of macrophage polarization. Stem Cells Transl Med. 2017;6:1018–28.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Nosbaum A, Prevel N, Truong H-A, Mehta P, Ettinger M, Scharschmidt TC, et al. Regulatory T cells facilitate cutaneous wound healing. J Immunol. 2016;196:2010–4.

    Article  CAS  PubMed  Google Scholar 

  48. Monguió-Tortajada M, Roura S, Gálvez-Montón C, Pujal JM, Aran G, Sanjurjo L, et al. Nanosized UCMSC-derived extracellular vesicles but not conditioned medium exclusively inhibit the inflammatory response of stimulated T cells: implications for nanomedicine. Theranostics. 2017;7:270–84.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  49. Hatanaka E, Monteagudo PT, Marrocos MS, Campa A. Neutrophils and monocytes as potentially important sources of proinflammatory cytokines in diabetes. Clin Exp Immunol. 2006;146:443–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. Yang J, Liu XX, Fan H, Tang Q, Shou ZX, Zuo DM, et al. Extracellular vesicles derived from bone marrow mesenchymal stem cells protect against experimental colitis via attenuating colon inflammation, oxidative stress and apoptosis. PLoS One. 2015;10:e0140551.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  51. Li X, Liu L, Yang J, Yu Y, Chai J, Wang L, et al. Exosome derived from human umbilical cord mesenchymal stem cell mediates MiR-181c attenuating burn-induced excessive inflammation. EBioMedicine. 2016;8:72–82.

    Article  PubMed  PubMed Central  Google Scholar 

  52. Yu B, Shao H, Su C, Jiang Y, Chen X, Bai L, et al. Exosomes derived from MSCs ameliorate retinal laser injury partially by inhibition of MCP-1. Sci Rep. 2016;6:34562.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. Ti D, Hao H, Tong C, Liu J, Dong L, Zheng J, et al. LPS-preconditioned mesenchymal stromal cells modify macrophage polarization for resolution of chronic inflammation via exosome-shuttled let-7b. J Transl Med. 2015;13:308.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  54. Ti D, Hao H, Fu X, Han W. Mesenchymal stem cells-derived exosomal microRNAs contribute to wound inflammation. Sci China Life Sci. 2016;59:1305–12.

    Article  CAS  PubMed  Google Scholar 

  55. Geiger A, Walker A, Nissen E. Human fibrocyte-derived exosomes accelerate wound healing in genetically diabetic mice. Biochem Biophys Res Commun. 2015;467:303–9.

    Article  CAS  PubMed  Google Scholar 

  56. Roy S, Sen CK. miRNA in wound inflammation and angiogenesis. Microcirculation. 2012;19:224–32.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  57. Midwood KS, Williams LV, Schwarzbauer JE. Tissue repair and the dynamics of the extracellular matrix. Int J Biochem Cell Biol. 2004;36(6):1031–7.

    Article  CAS  PubMed  Google Scholar 

  58. Singer AJ, Raf C. Mechanisms of disease: cutaneous wound healing. N Engl J Med. 1999;341:738–46.

    Article  CAS  PubMed  Google Scholar 

  59. Arnold F, West DC. Angiogenesis in wound healing. Pharmacol Ther. 1991;52:407–22.

    Article  CAS  PubMed  Google Scholar 

  60. Sahoo S, Klychko E, Thorne T, Misener S, Schultz KM, Millay M, et al. Exosomes from human CD34+ stem cells mediate their pro-angiogenic paracrine activity. Circ Res. 2011;109:724–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  61. Li X, Jiang C, Zhao J. Human endothelial progenitor cells-derived exosomes accelerate cutaneous wound healing in diabetic rats by promoting endothelial function. J Diabetes Complicat. 2016;30:986–92.

    Article  Google Scholar 

  62. Liu Y, Min D, Bolton T, Nubé V, Twigg SM, Yue DK, et al. Increased matrix metalloproteinase-9 predicts poor wound healing in diabetic foot ulcers. Diabetes Care. 2009;32:117–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  63. Diegelmann Robert F, Evans Melissa C. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci. 2004;9:283–9.

    Article  CAS  PubMed  Google Scholar 

  64. Hu L, Wang J, Zhou X, et al. Exosomes derived from human adipose mensenchymal stem cells accelerates cutaneous wound healing via optimizing the characteristics of fibroblasts. Sci Rep. 2016;6:32993.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  65. Shabbir A, Cox A, Rodriguez-Menocal L, Salgado M, Van Badiavas E. Mesenchymal stem cell exosomes induce proliferation and migration of normal and chronic wound broblasts and enhance angiogenesis in vitro. Stem Cells Dev. 2015;24(14):1635–47.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  66. Kim YJ, Yoo SM, Park HH, Lim HJ, Kim YL, Lee S, et al. Exosomes derived from human umbilical cord blood mesenchymal stem cells stimulates rejuvenation of human skin. Biochem Biophys Res Commun. 2017;493(2):1102–8.

    Article  CAS  PubMed  Google Scholar 

  67. Guo S, Dipietro LA. Factors affecting wound healing [J]. J Dent Res. 2010;89(3):219–29.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  68. Gospodarowicz D. Biological activities of fibroblast growth factors [J]. Ann N Y Acad Sci. 1991;638:1–8.

    Article  CAS  PubMed  Google Scholar 

  69. Zhang B, Wang M, Gong A, Zhang X, Wu X, Zhu Y, et al. HucMSC-exosome mediated-Wnt4 signaling is required for cutaneous wound healing. Stem Cells. 2015;33(7):2158–68.

    Article  CAS  PubMed  Google Scholar 

  70. Zhang B, Wu X, Zhang X, Sun Y, Yan Y, Shi H, et al. Human umbilical cord mesenchymal stem cell exosomes enhance angiogenesis through the Wnt4/β-catenin pathway.[J]. Stem Cells Transl Med. 2015;4:513–22.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  71. Zhao B, Zhang Y, Han S, Zhang W, Zhou Q, Guan H, et al. Exosomes derived from human amniotic epithelial cells accelerate wound healing and inhibit scar formation. J Mol Histol. 2017;48:121–32.

    Article  CAS  PubMed  Google Scholar 

  72. Zhang J, Guan J, Niu X,  Hu G, Guo S, Li Q, et al. Exosomes released from human induced pluripotent stem cells-derived MSCs facilitate cutaneous wound healing by promoting collagen synthesis and angiogenesis. J Transl Med. 2015;13:49.

  73. Kou X, Xu X, Chen C, Sanmillan ML, Cai T, Zhou Y, et al. The Fas/Fap-1/Cav-1 complex regulates IL-1RA secretion in mesenchymal stem cells to accelerate wound healing. Sci Transl Med. 2018;10:432.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  74. Fang S, Xu C, Zhang Y,  Xue C, Yang C, Bi H, et al. Umbilical cord-derived mesenchymal stem cell-derived exosomal micrornas suppress myofibroblast differentiation by inhibiting the transforming growth factor- beta/smad2 pathway during wound healing. Stem Cells Transl Med. 2016;5(10):1425–39.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  75. Wang L, Hu L, Zhou X, Xiong Z, Zhang C, Shehada HMA, et al. Exosomes secreted by human adipose mesenchymal stem cells promote scarless cutaneous repair by regulating extracellular matrix remodelling. Sci Rep. 2017;7(1):13321.

  76. Liu X, Wang Z, Wang R, Zhao F, Shi P, Jiang Y, et al. Direct comparison of the potency of human mesenchymal stem cells derived from amnion tissue, bone marrow and adipose tissue at inducing dermal fibroblast responses to cutaneous wounds. Int J Mol Med. 2013;31:407–15.

    Article  PubMed  CAS  Google Scholar 

  77. Hass R, Kasper C, Böhm S, Jacobs R. Different populations and sources of human mesenchymal stem cells (MSC): a comparison of adult and neonatal tissue-derived MSC. Cell Commun Signal. 2011;9:12.

  78. Kusuma GD, Carthew J, Lim R, Frith JE. Effect of the microenvironment on Mesenchymal stem cell paracrine signaling: opportunities to engineer the therapeutic effect. Stem Cells Dev. 2017;26(9):617–31.

    Article  CAS  PubMed  Google Scholar 

  79. Syva SH, Ampon K, Lasimbang H, Fatimah SS. Microenvironmental factors involved in human amnion mesenchymal stem cells fate decisions. J Tissue Eng Regen Med. 2017;11(2):311–20.

    Article  PubMed  CAS  Google Scholar 

  80. Lee DE, Ayoub N, Agrawal DK. Mesenchymal stem cells and cutaneous wound healing: novel methods to increase cell delivery and therapeutic efficac. Stem Cell Res Ther. 2016;7:37.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  81. Ho IA, Toh HC, Ng WH, Teo YL, Guo CM, Hui KM, et al. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis. Stem Cells. 2013;31(1):146–55.

    Article  CAS  Google Scholar 

  82. Lee JK, Park SR, Jung BK, Jeon YK, Lee YS, Kim MK, et al. Exosomes derived from mesenchymal stem cells suppress angiogenesis by down-regulating VEGF expression in breast cancer cells. PLoS One. 2013;8(12):e84256.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  83. Yang X, Li X, Xiao J. Exosomes secreted from IFN-γ prestimulated hUC-MSCs induce regulatory T cells. Chin Pharmacol Bull. 2017;33(1):45–51.

  84. Madrigal M, Rao KS, Riordan NH. A review of therapeutic effects of mesenchymal stem cell secretions and induction of secretory modification by different culture methods. J Transl Med. 2014;12:260.

    Article  PubMed  PubMed Central  Google Scholar 

Download references


Not applicable.


PH reviewed the literature, interpreted the information, and drafted the review. QY, QW, QS helped create the figures and compiled the reference list. IDP and AC provided the recommendations. DW and UA provided the recommendations and helped draft and revise the review. All authors read and approved the final manuscript.


This article is funded by National Natural Science Foundation of China (81871570, and 81560313).

Author information

Authors and Affiliations



Peng Hu, PhD, is surgeon at Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, China. His research interests include biomaterials for tissue engineering and wound repair.

Qinxin Yang, Qi Wang and Chenshuo Shi, are postgraduates at Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, China. Their research interest is the mesenchymal stromal cell in wound repair.

Dali wang, MD, Professor, is the Academic Leader at Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, China. His research interests include wound repair, prevention and treatment of scar.

Ubaldo Armato, PhD, MD, Professor, is the Academic Leader at Human Histology and Embryology Unit, University of Verona Medical School, Italy. His research interests include biomaterials for tissue engineering, Alzheimer’s Disease, burn wounds repair and Clinical proteomics.

Ilaria Dal Prà, PhD, Professor, is director at Human Histology and Embryology Unit, University of Verona Medical School, Italy. Her research interests include Alzheimer’s Disease, biomaterials for tissue engineering and Clinical proteomics.

Anna Chiarini, PhD, Professor, is researcher at Human Histology and Embryology Unit, University of Verona Medical School, Italy. Her research interests include Clinical proteomics, Alzheimer’s Disease and biomaterials for tissue engineering.

Corresponding authors

Correspondence to Dali Wang or Ubaldo Armato.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hu, P., Yang, Q., Wang, Q. et al. Mesenchymal stromal cells-exosomes: a promising cell-free therapeutic tool for wound healing and cutaneous regeneration. Burn Trauma 7, 38 (2019).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: